cd3 rabbit anti human antibody  (Servicebio Inc)

Journal: Translational Cancer Research

Article Title: OX40L and IL-2 combination strategy for gastric cancer immunotherapy

doi: 10.21037/tcr-2025-707

Figure Lengend Snippet: Immunohistochemical and flow cytometric analysis of gastric cancer tissue. (A) H&E histochemical staining of tumor-free area (left), tumor margin (middle), and gastric tumor site (right). (B) Representative IHC images for CD3 + , CD4 + , CD8 + , FOXP3, OX40, and OX40L in tumor-free area (left column), tumor margin (middle column), and gastric tumor site (right column) sections. (C) Immunofluorescence staining for the co-expression of CD3 + and OX40 in gastric cancer with anti-CD3 and anti-OX40 antibodies and DAPI for nuclear staining. (D) Flow cytometry analysis of OX40 expression in CD3 cells obtained from PBMC of gastric cancer patients (upper panel) and OX40 expression on CD3 on TILs in gastric cancer patients (low panel). Statistical analysis of all data were performed using one-way ANOVA. Data are shown as mean ± standard deviation. ns, nonsignificant; *, P<0.05; ***, P<0.001. ANOVA, analysis of variance; DAPI, 4',6-diamidino-2-phenylindole; H&E, hematoxylin & eosin; IHC, immunohistochemistry; OX40L, OX40 ligand; PBMC, peripheral blood mononuclear cells; TILs, tumor-infiltrating lymphocytes.

Article Snippet: Immunohistochemistry (IHC) analyses were performed using specific primary rabbit anti-human CD3 monoclonal antibody (Proteintech Group, Wuhan, China), mouse anti-human OX40 and rabbit anti-human OX40L monoclonal antibodies (CST, Danvers, MA, USA), and rabbit anti-human CD4 (Proteintech Group, Wuhan, China), mouse anti-human CD8 (Proteintech Group, Wuhan, China) antibody, rabbit anti-human FOXP3 monoclonal antibodies (CST, Danvers, MA, USA).

Techniques: Immunohistochemical staining, Staining, Immunofluorescence, Expressing, Flow Cytometry, Standard Deviation, Immunohistochemistry

Journal: Cell Reports Medicine

Article Title: Dissecting the immune landscape in pediatric high-grade glioma reveals cell state changes under therapeutic pressure

doi: 10.1016/j.xcrm.2025.102095

Figure Lengend Snippet: Integrative comparison of myeloid and T cells across pediatric and adult HGG (A) Proportion of each T cell subpopulation by age group and CD4/CD8 annotation. Asterisks denote significant changes in observed proportion ( p ≤ 0.05). Adult tumor-associated myeloid cells from the study by Neftel et al. and Venteicher et al. , (B) Average marker gene expression from dysfunctional, predysfunctional program-associated, and cytotoxic CD8 + T cells in indicated age groups. (C) UMAP of all myeloid cells, colored by age group (adult tumor-associated myeloid cells from the study by Neftel et al. and Venteicher et al. , ). No integration by Harmony was performed here. (D) Proportion of each myeloid subpopulation by age group and subtype. Asterisks denote significant observed changes in proportion compared to pediatric patients ( p ≤ 0.05). Statistical significance assessed using the propeller function from the R package speckle. (E) Proportion of myeloid cell subpopulations, represented by each individual expression program, by subtype and age group. Asterisks denote published datasets. (F) Pearson correlation and the number of overlapping genes between programs shared by adult and pediatric T cells (top) and myeloid cells (bottom). (G) Percentage of clonally expended T cells detected per sample in our pHGG and adult HGG cohort. Asterisk denotes significant change assessed via Student’s t test. Data are represented as mean ± SEM. (H) Heatmap depicting T cell-specific genes ranked according to their expression value among all detected transcripts detected in T cells in the pHGG cohort. (I) Relative expression of SELPLG and KLRB1 mRNAs in T cells from our pHGG and adult HGG cohorts. (J) Flow cytometric analysis for CD162 and PD-1 expression on the surface of T cells (CD3 + ) from a representative sample from this pHGG cohort.

Article Snippet: Anti-human CD3 (D7A6E) , Cell Signaling , Cat# 85061; RRID: AB_2721019.

Techniques: Comparison, Marker, Gene Expression, Expressing

Journal: Pediatric Rheumatology

Article Title: Fasciitis-panniculitis syndrome with autoantibodies reacting to adipocyte pericellular fibers: a case report

doi: 10.1186/s12969-025-01071-w

Figure Lengend Snippet: Biopsy findings. a : A representative hematoxylin and eosin (HE)-stained section of the patient’s fat tissue showing thickened collagen bundles (×40). b : A HE-stained section of the patient’s en bloc biopsy showing infiltration of inflammatory cells into the adipose tissue and necrotizing vasculitis (×100). c : A HE-stained section showing edema and diffuse infiltration of inflammatory cells into the perifascia with fibrinoid necrosis of the vascular wall (×100). d : A phosphotungstic acid hematoxylin (PTAH)-stained section showing fibrin deposition in the vessel wall (×200). e : Immunohistochemical staining showing infiltration of CD3 + T lymphocytes into the perivascular area of the adipose septa (×100). Anti-human CD3 rabbit antibody was obtained from Cell Signaling Technology (Danvers, MA, USA). HRP-conjugated anti-rabbit IgG Novolink polymer (Leica Biosystems, Nussloch, Germany), was used as a secondary antibody. f : Immunohistochemical staining showing less dominant infiltration of CD19 + B lymphocytes into the perivascular area of the adipose septa (×100). Anti-CD19 rabbit antibody was obtained from Cell Signaling Technology and used along with the above Novolink polymer. g : Immunohistochemical staining showing infiltration of CD68 + macrophages into the perivascular area of the adipose septa (×100). Anti-CD68 antibody [PG-M1] was obtained from abcam. Alkaline phosphatase-conjugated anti-mouse IgG Histofine Simple Stain (Nichirei) was used as a secondary antibody. h : A HE-stained section showing unaffected muscle tissue in the same biopsy (×100)

Article Snippet: Anti-human CD3 rabbit antibody was obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Staining, Immunohistochemical staining, Polymer